TABLE E-1 Specific Primers Sequence Gene Annealing Temperature Application of Primer Forward and Reverse Primers (5´→ 3´) 50
![Phases of competitor DNA construction. F. forward primer obtained in... | Download Scientific Diagram Phases of competitor DNA construction. F. forward primer obtained in... | Download Scientific Diagram](https://www.researchgate.net/publication/266284183/figure/fig1/AS:641700728942595@1530004784379/Phases-of-competitor-DNA-construction-F-forward-primer-obtained-in-Neilan-et-al.png)
Phases of competitor DNA construction. F. forward primer obtained in... | Download Scientific Diagram
![Positions of primers-OF (outer forward), F (forward), R (reverse), and... | Download Scientific Diagram Positions of primers-OF (outer forward), F (forward), R (reverse), and... | Download Scientific Diagram](https://www.researchgate.net/publication/14186676/figure/fig2/AS:667653278556168@1536192354575/Positions-of-primers-OF-outer-forward-F-forward-R-reverse-and-CR-control.png)
Positions of primers-OF (outer forward), F (forward), R (reverse), and... | Download Scientific Diagram
![SOLVED: You have a forward primer that anneals within sticky fingers and reverse primer that anneals within the Pen-tag (see pictures below). Xbal Promoter EcoRI Xhol Forward Sall primer Pst1 Tbdl Sticky SOLVED: You have a forward primer that anneals within sticky fingers and reverse primer that anneals within the Pen-tag (see pictures below). Xbal Promoter EcoRI Xhol Forward Sall primer Pst1 Tbdl Sticky](https://cdn.numerade.com/ask_images/a6e13a5bf67a46fdb6b1d014638e12cd.jpg)
SOLVED: You have a forward primer that anneals within sticky fingers and reverse primer that anneals within the Pen-tag (see pictures below). Xbal Promoter EcoRI Xhol Forward Sall primer Pst1 Tbdl Sticky
![PDF] Primers used for RT-PCR and RT-qPCR gene expression analysis Gene symbol Forward primer Reverse primer Abcc 2 | Semantic Scholar PDF] Primers used for RT-PCR and RT-qPCR gene expression analysis Gene symbol Forward primer Reverse primer Abcc 2 | Semantic Scholar](https://d3i71xaburhd42.cloudfront.net/e5c8b846467803aebbd17d1e8e6bc53e8e4f2045/3-Table3-1.png)